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Ideal for grinding hard, brittle samples to an analytical fineness, slurry grinding, blending powders and mixing emulsions: Height (English) 3 in. Includes: Screw on cap with O-ring, two 0.5 in. and four 0.25 in. Stainless Steel balls: Length (Metric) 7.62cm: Load Capacity (Metric) Centralized: 25mL: Sample Volume (English) 0.1 to 0.33 oz. Size ...

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The eukaryotic replisome is a critical determinant of genome integrity with a complex structure that remains poorly characterized. A central unresolved issue is how the Cdc45-MCM-GINS helicase is linked to DNA polymerase epsilon, which synthesizes the leading strand at replication forks and is an important focus of regulation.

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Briefly, the steps involved were as follows: An aliquot of fresh PCR product was mixed with molecular biology grade distilled water to 4μl and taken in a microfuge tube. 1μl of salt solution containing 1.2M Sodium chloride and 0.06M Magnesium chloride mixture was added to it followed by lμl of TOPO vector to make up the final volume to 6μl.

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The CIII protein encoded by the temperate coliphage lambda acts as an inhibitor of the ubiquitous Escherichia coli metalloprotease HflB (FtsH). This inhibition results in the stabilization of transcription factor λCII, thereby helping the phage to lysogenize the host bacterium. λCIII, a small (54-residue) protein of unknown structure, also protects σ32, another specific substrate of HflB.

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Plant Molecular Biology Reporter 11, 333–337. Saini, H., Shepherd, M. and Henry, R.J. (1999) Microwav e extraction of total genomic DNA from barley grains for use in PCR.

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2.4. Molecular Characterization and Phylogenetic Analysis of bla TEM Genes. Amplification of bla TEM gene was performed in 96-well thermal cycler (Applied Biosystems, USA) with primers: F: 5′-ATGAGTATTCAACATTTTCGTC-3′; R: 5′-TTACCAATGCTTAATCAGTGAG-3′, generated by using Primer3 (version 0.4.0) [].Briefly, each reaction was carried out in 20 μL reaction volume using 1x …

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Briefly, the steps involved were as follows: An aliquot of fresh PCR product was mixed with molecular biology grade distilled water to 4μl and taken in a microfuge tube. 1μl of salt solution containing 1.2M Sodium chloride and 0.06M Magnesium chloride mixture was added to it followed by lμl of TOPO vector to make up the final volume to 6μl.

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Molecular biology is the branch of biology that deals with the structure and function of the macromolecules essential to life such as DNA, RNA, proteins etc. It overlaps with biology and chemistry and in particular, genetics and biochemistry. Now, the molecular basis of human disease is better characterized and it is

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